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lambda apc vio770 miltenyi  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec lambda apc vio770 miltenyi
    Lambda Apc Vio770 Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+light+chain+antibody+/Ig+%CE%BB+Light+Chain+Antibody%2C+anti-human/pm41963633-395-80-82
    Average 91 stars, based on 8 article reviews
    lambda apc vio770 miltenyi - by Bioz Stars, 2026-11
    91/100 stars

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    Related Articles

    Incubation:

    Article Title: Blocking α 4 β 7 integrin delays viral rebound in SHIV SF162P3 -infected macaques treated with anti-HIV broadly neutralizing antibodies
    Article Snippet: .. Plates were washed and incubated with anti-Ig human lambda light chain–biotin (Miltenyi Biotec), which does not recognize the kappa chain of the Rh-α 4 β 7 for 1 hour at room temperature. .. Plates were washed and incubated with diluted streptavidin-HRP (Invitrogen) for 1 hour at room temperature.

    Article Title: Safety and Immunological Evaluation of Interleukin-21 Plus Anti-α4β7 mAb Combination Therapy in Rhesus Macaques
    Article Snippet: .. After 1-h incubation at RT, plates were washed six times with wash buffer followed by the addition of 100 μL of a 1:100 dilution of a monoclonal anti–lambda light chain-biotin (clone IS7-24C7; Miltenyi Biotech, Cologne, Germany) per well. .. After 1-h incubation at RT, plates were washed six times with wash buffer followed by the addition of 100 μL of a 1:10,000 dilution of streptavidin–horseradish peroxidase (HRP) (Invitrogen) per well.

    Staining:

    Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
    Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.

    Amplification:

    Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
    Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.

    Magnetic Cell Separation:

    Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
    Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.

    FACS:

    Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
    Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.

    other:

    Article Title: Preclinical safety and efficacy of a therapeutic antibody that targets SARS-CoV-2 at the sotrovimab face but is escaped by Omicron
    Article Snippet: Anti-Lambda-PE (clone IS7-24C) , Miltenyi Biotec , Cat# 130-119-778; RRID: AB_2751837.




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    SPE and immunofixation confirming monoclonal gammopathy. (A) SPE demonstrating an M-spike in the γ region. (B) Immunofixation electrophoresis revealing a monoclonal IgA λ pattern, confirming the presence of IgA-λ type multiple myeloma. (C) Immunofixation electrophoresis further confirming the monoclonal IgA λ pattern. AU, arbitrary units; SPE, serum protein electrophoresis. ELP, electrophoresis reference; G, IgG; A, IgA; M, IgM; K, κ light chain; L, <t>λ</t> <t>light</t> chain; D, IgD; E, IgE.
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    Image Search Results


    SPE and immunofixation confirming monoclonal gammopathy. (A) SPE demonstrating an M-spike in the γ region. (B) Immunofixation electrophoresis revealing a monoclonal IgA λ pattern, confirming the presence of IgA-λ type multiple myeloma. (C) Immunofixation electrophoresis further confirming the monoclonal IgA λ pattern. AU, arbitrary units; SPE, serum protein electrophoresis. ELP, electrophoresis reference; G, IgG; A, IgA; M, IgM; K, κ light chain; L, λ light chain; D, IgD; E, IgE.

    Journal: Experimental and Therapeutic Medicine

    Article Title: A rare dermatological manifestation of follicular spicules in a patient with multiple myeloma and end-stage renal disease on hemodialysis: A case report

    doi: 10.3892/etm.2025.12930

    Figure Lengend Snippet: SPE and immunofixation confirming monoclonal gammopathy. (A) SPE demonstrating an M-spike in the γ region. (B) Immunofixation electrophoresis revealing a monoclonal IgA λ pattern, confirming the presence of IgA-λ type multiple myeloma. (C) Immunofixation electrophoresis further confirming the monoclonal IgA λ pattern. AU, arbitrary units; SPE, serum protein electrophoresis. ELP, electrophoresis reference; G, IgG; A, IgA; M, IgM; K, κ light chain; L, λ light chain; D, IgD; E, IgE.

    Article Snippet: Primary antibodies were incubated overnight at 4 ̊C, including: CD138 (cat. no. ab34164; dilution 1:100; Abcam), CD56 (cat. no. ab75813; dilution 1:200; Abcam), κ light chain (cat. no. ZM-0317; dilution 1:150; OriGene Technologies, Inc.) and λ light chain (cat. no. ZM-0318; dilution 1:150; Origene Technologies, Inc.).

    Techniques: Electrophoresis, Protein Electrophoresis

    Immunohistochemical characterization of bone marrow biopsy. (A) Congo red staining showed amyloid deposits with birefringence under polarized light, confirming amyloidosis. (B) Scattered CD3 + T cells. (C) CD20 + B cells. (D) Diffuse CD56 positivity indicating natural killer cell involvement. (E) Diffuse CD138 staining marking plasma cell infiltration. (F) Negative κ light chain staining. (G) Diffuse positive staining of λ light chains, confirming monoclonality. (H) Multiple myeloma oncogene 1-positivity indicated plasma cell differentiation. (I) Hematoxylin and eosin staining revealed diffuse infiltration by atypical lymphoid/plasma cells. (J) Positive staining for amyloid deposits in bone marrow biopsy. All images were captured under a light microscope at x400 magnification.

    Journal: Experimental and Therapeutic Medicine

    Article Title: A rare dermatological manifestation of follicular spicules in a patient with multiple myeloma and end-stage renal disease on hemodialysis: A case report

    doi: 10.3892/etm.2025.12930

    Figure Lengend Snippet: Immunohistochemical characterization of bone marrow biopsy. (A) Congo red staining showed amyloid deposits with birefringence under polarized light, confirming amyloidosis. (B) Scattered CD3 + T cells. (C) CD20 + B cells. (D) Diffuse CD56 positivity indicating natural killer cell involvement. (E) Diffuse CD138 staining marking plasma cell infiltration. (F) Negative κ light chain staining. (G) Diffuse positive staining of λ light chains, confirming monoclonality. (H) Multiple myeloma oncogene 1-positivity indicated plasma cell differentiation. (I) Hematoxylin and eosin staining revealed diffuse infiltration by atypical lymphoid/plasma cells. (J) Positive staining for amyloid deposits in bone marrow biopsy. All images were captured under a light microscope at x400 magnification.

    Article Snippet: Primary antibodies were incubated overnight at 4 ̊C, including: CD138 (cat. no. ab34164; dilution 1:100; Abcam), CD56 (cat. no. ab75813; dilution 1:200; Abcam), κ light chain (cat. no. ZM-0317; dilution 1:150; OriGene Technologies, Inc.) and λ light chain (cat. no. ZM-0318; dilution 1:150; Origene Technologies, Inc.).

    Techniques: Immunohistochemical staining, Staining, Clinical Proteomics, Cell Differentiation, Light Microscopy

    Flow cytometric immunophenotyping of bone marrow cells from a patient with PCM. (A) Lymphocyte analysis showing a decreased CD4/CD8 ratio. (B and C) Quantification of CD57⁺ LGLs, demonstrating a normal proportion of this subset. (D) Marked expansion of plasma cells detected within the bone marrow aspirate. (E) Dual-parameter plot of CD38 and CD45 expression revealing a distinct CD38 bright+ /CD45 dim+ population, accounting for 33.19% of total nucleated cells, consistent with malignant plasma cell infiltration. (F) Intracellular immunoglobulin light chain staining within the CD38 bright+ population indicating prominent λ light chain restriction (97.37%) with minimal κ expression (0.03%), confirming clonal proliferation and supporting the diagnosis of PCM. LGLs, large granular lymphocytes; NC, nucleated cell; PCM, plasma cell myeloma; A, allophycocyanin-Alexa Fluor 700; APC, allophycocyanin; CD38bri + , CD38 bright positive; DNT, double negative T cells; DPT, dual-parameter plot; ECD, energy-coupled dye; H, height parameter; KO525, Krome Orange 525; Lym, lymphocytes; NK, natural killer cells; PC5.5, phycoerythrin-cyanine 5.5; PE, phycoerythrin; SSC, side scatter.

    Journal: Experimental and Therapeutic Medicine

    Article Title: A rare dermatological manifestation of follicular spicules in a patient with multiple myeloma and end-stage renal disease on hemodialysis: A case report

    doi: 10.3892/etm.2025.12930

    Figure Lengend Snippet: Flow cytometric immunophenotyping of bone marrow cells from a patient with PCM. (A) Lymphocyte analysis showing a decreased CD4/CD8 ratio. (B and C) Quantification of CD57⁺ LGLs, demonstrating a normal proportion of this subset. (D) Marked expansion of plasma cells detected within the bone marrow aspirate. (E) Dual-parameter plot of CD38 and CD45 expression revealing a distinct CD38 bright+ /CD45 dim+ population, accounting for 33.19% of total nucleated cells, consistent with malignant plasma cell infiltration. (F) Intracellular immunoglobulin light chain staining within the CD38 bright+ population indicating prominent λ light chain restriction (97.37%) with minimal κ expression (0.03%), confirming clonal proliferation and supporting the diagnosis of PCM. LGLs, large granular lymphocytes; NC, nucleated cell; PCM, plasma cell myeloma; A, allophycocyanin-Alexa Fluor 700; APC, allophycocyanin; CD38bri + , CD38 bright positive; DNT, double negative T cells; DPT, dual-parameter plot; ECD, energy-coupled dye; H, height parameter; KO525, Krome Orange 525; Lym, lymphocytes; NK, natural killer cells; PC5.5, phycoerythrin-cyanine 5.5; PE, phycoerythrin; SSC, side scatter.

    Article Snippet: Primary antibodies were incubated overnight at 4 ̊C, including: CD138 (cat. no. ab34164; dilution 1:100; Abcam), CD56 (cat. no. ab75813; dilution 1:200; Abcam), κ light chain (cat. no. ZM-0317; dilution 1:150; OriGene Technologies, Inc.) and λ light chain (cat. no. ZM-0318; dilution 1:150; Origene Technologies, Inc.).

    Techniques: Clinical Proteomics, Expressing, Staining, Biomarker Discovery